TLC scan of the gut content amino acids of larval instars (Ist to IVth) of Callosobruchus chinensis Linn. infesting moong.

Pushpalata Hansdak^a*, Rani Srivastaava^b & M.Prasad^a
aDepartment of Zoology, Ranchi University, Ranchi, Jharkhand, India
^bDept. of Zoology, B.M.D.College, Dayalpur, BRA Bihar University, Muzaffarpur, Bihar

Received  06th Jan.,2012; Revised 15th June.,2012

Abstract : The TLC scan of the gut content amino acids of C.chinensis larval instars (I-IV) has provided an unique picture of moong host specific biomolecular profile. The gut content mashurrette in 80% methanol as the extraction solvent was processed through the Thin Layer Chromatography (Willson & Walker, 1999, Sharma, 2003, Bobbit,J. and M. Mikes, 1972, Bobbit, S. & P. Mikes. 1998) for accurate separation & isolation of amino acids. The no. of isolated spots in the gut content shows instar specific (I-IV) variation due to the enzymatic constitution of the developing larval gut which is responsible for breakdown and synthesis ( metabolism) of the biomolecules, procured from the host protein.  All plates were exposed to the standard BAW separation solvent and 2% Nin-hydrin as location  reagent.