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Prevalence of antibiotic resistance pattern of bacteria isolated from urinary tract infection

Nirupama Bhimania, Riteshkumar Aryaa, Prachi Davea, Bhavna Chaudharya, Komalben Hiranib & Navneet Kumar Singhc*
aDepartment of Microbiology, Mehsana Urban Institute of Sciences, Ganpat University, Mehsana, Gujarat, India.
bSchool of Biological Engineering & Life Sciences, Shobhit Institute of Engineering & Technology,
Deemed to-Be-University, Modipuram, Meerut, India
cDepartment of Paramedical Sciences, Sumandeep Vidyapeeth Deemed University, Gujarat, India.
Received : 16th July, 2022 ; Revised : 16th August, 2022

Abstract– The present study was aimed to determine the resistance framework of pathogenic bacteria against common antibiotics isolated from the urine samples of patients suffering from an infection of urinary tract. The work was initiated by using 5 urine samples collected from the Pathology Laboratory of JNU Hospital, Jaipur. From those samples, 16 isolates were differentiated based on their morphology and biochemical characteristics. After tentative identification of isolates, an antibiotic sensitivity test was performed on those isolates by using the Kirby Buaer method and susceptibility profiles were identified using the minimum inhibitory concentration (MIC). The male and female both are infected with pathogenic bacteria which cause UTI. Biochemical reactions depicted that patients infected with bacteria were Escherichia coli, Enterobacter spp., Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus spp., Streptococcus spp. and Enterococcus spp. Based on the antibiotic susceptibility test using eight different antibiotics, three different isolates were found to be resistant to these antibiotics. Trimethoprim (TR-30), Gentamycin (HLG-120), Amoxicillin (AMX-10), Penicillin G (P-1), Ceftazidime (CAZ-30), Ciprofloxacin (CIP-5) and Erythromycin (E-5) were used for this study. This study exhibited the resistance rate of isolates against antibiotics which is a concern of worry for society.

Key words: UTI (Urinary Tract Infection), Multidrug resistance, antibiotics, pathogenic bacteria.

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