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DEHP induced reproductive toxicity and oxidative stress in male rats

Kumari Gouria*, Preety Sinhab & Anuradha Prakashb
aUniversity Department of Zoology, Magadh University, Bodh Gaya, Bihar, India.
bP.G. Department of Zoology, Anugrah Narayan College, Patna, Bihar, India.
Received : 20th July, 2022 ; Revised : 21st August, 2022

Abstract– DEHP is a well-known chemical of commercial importance present everywhere around us. It has become a chemical of concern due to its high chances of exposure as it is present in quite huge amounts in our environment and its damaging effects reported in various living system. Present study is planned to see how it damages male reproductive system in animal model using Wistar Rat exposed to 1000mg/kg body weight of daily dose for 4, 8, 12 and 16 weeks. This resulted in significant accumulation of the chemical in the testis of rat which showed increase in amount with the increase of period of exposure. This study also showed similar trend as the tissue level accumulation of DEHP, viz. oxidative DNA damage of the testis tissue as shown by the measure of 8-OHdG formed in the DNA of testis tissue due to DEHP exposure was increasing significantly with exposure duration similarly lipid peroxidation level of the serum sample of exposed rat was significantly high in comparisons to that of control group rats and increased significantly with increasing exposure interval. These findings clearly suggest that DEHP by accumulating in the tissue reaches to cells and damages DNA at various levels. Whereas DNA of testis showed high level of oxidative damage, serum of the exposed rat also showed high level of lipid peroxidation, showing it causes oxidative stress in the animal damaging the reproductive system significantly.

Key words: DEHP, Testis, DNA damage, Oxidative Stress, HPLC, rat 8-OHdG

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