Press "Enter" to skip to content

Detection of mucopolysacharides by PAS technique in the fore-gut of larval instars of gram beetle, Callosobruchus chinensis Linn.

Pushpalata Hansdak*
Department of Zoology, Ranchi University, Ranchi,Jharkhand, India

Received : 25th November, 2015: Revised : 17th February, 2016

Abstract : PAS histochemical detection and localization of mucopolysacharide in the fore-gut of larval instars of gram beetle Callosobruchus chinensis Linn. has not been reported so far. Although mucopolysacharides are biochemically high energy structural and functional biomolecules which contribute in the organization of mucus and peritrophic membranes, organdies and other important cellular structures in combination with protein yet the amount of mucopolysacharide deposition in cells has been found to be very less as because they are metabolically more functional for energy production. Therefore, sparsed miganata or pinkish purple patches have been observed by PAS (Periodic Acid Schiff’s reagent) staining technique which is very much popular histochemical method to detect differential degree of mucopolysacharides and other polysacharides present in the cells. The larval instars I-V collected from lab. culture on common gram, Cicer arietinum L. jars were sacrificed to take out their foregut for further fixation, microtomy and histochemistry which gave significant results.

Be First to Comment

Leave a Reply