B. Chand*, Rizwan Ahmad, Prakash Singh, M. P. Sinha & S. K. Shrivastava
Dept. of Zoology, Patna University, Patna.
Received 9th June 2007; Revised 12th August 2007
Abstract : Healthy groups of Clarias batrachus ranging from 100 to 110 ± 10 gms and size 5-6″ were collected, disinfected with KMnO4 solution and acclimated under ideal laboratory condition. The LC50 of commercial grade Rogor (EC30%) was determined by standard APHA method. Fishes were exposed to 2 ppm, 4 ppm and 6 ppm rogor for 2, 4 & 8 days respectively. Rogor exposure on fishes showed great fluctuation in serum SGPT, SGOT, ALP and ACP respectively. At 2 ppm concentration SGPT initially declines then shoots up within 8 days, but at 4 ppm and 6 ppm concentration SGPT shoots up just within 4 days after rogor exposure (P<0.05). Serum SGOT shows initial decline after 2 days exposure of 2 ppm rogor pointing towards some stress response in fish, but at higher doses SGOT shoots up within 4 days (P<0.05). Serum ALP shows 2 fold rise in just 4 days treatment of 2 ppm rogor (P<0.05) whereas higher doses i.e. 6 ppm ALP shows a significant rise in both shorter and longer duration (P<0.001). Serum ACP shows a general trend of rising in all the three doses of rogor exposure and is highly significant at 4 ppm rogor concentration (P<0.05).