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Molecular identification of Fusarium wilt pathogens of Psidium guajava L. using ITS marker

V.K. Gupta*a, A.K. Misrab, S. Sharmaa, P.K. Jaina, S. Roya, D. Diwana, B. Chakrabortya, & S. Paliwala
aDepartment of Biotechnology, MITS University, Lakshmangarh, Rajasthan, India
bCentral Institute for Subtropical Horticulture, P.O.Kokori, Rahmankhera, Lucknow-227 017, UP, India.
Received 14th December, 2009; Revised 18th February, 2010

Abstract : Fusarium spp. causes severe wilt disease to Psidium guajava L. in India. Isolates of Fusarium can differ in virulence, however virulence and colony morphology are not correlated. 42 isolates, each of  Fusarium, oxysporum f. sp. psidii and Fusarium solani, morphologically indistinguishable. These isolate were characterized by ITS (internal transcribed spacer) marker. ITS 4 marker showed specificity for genus Fusarium, Approximately product size of 210 bp for Fusarium oxysporum f. sp. psidii and 250 bp was obtained for Fusarium solani after successful amplification. The assay is useful for rapid identification for Fusarium spp. isolates causing wilt disease in Psidium guajava L and amplification of these ITS marker technologies can be used for early diagnosis of the wilt infected Psidium guajava L. Plants.


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