Chahat Kausar* & Hanuman Prasad Sharma
University Department of Botany, Ranchi University, Ranchi, Jharkhand, India.
Received : 16th July, 2022 ; Revised : 16th August, 2022
Abstract– Phytochemicals are plant products produced as a result of primary or secondary metabolism which is used by plants for various purposes such as pigment or as a defence against insects and predators. Phytochemicals are being increasingly utilized for medicinal purposes due to the presence of bioactive compounds in them. The phytochemical analysis of the leaf extracts was performed both qualitatively and quantitatively with the respective methods and the differences in the phytochemical status of both the plant extracts were determined. The anti-oxidative capacity of the leaf extracts was measured by DPPH radical scavenging activity. The presence and characterization of bioactive active compounds were assessed by Thin Layer Chromatography. The anti-tumorigenic potential of the leaf extracts was assessed by the tetrazolium dye method viz. MTT assay. The anti-tumorigenic potential of the leaf extracts was analyzed in three colon cancer cell lines viz., HCT-15, COLO-205, and HT-29 and in normal human keratinocyte (HaCaT) cell line.
Key words: Phytochemicals, 2,2, Diphenyl picryl hydrazine, MTT, inhibitory concentration, TLC
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