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Antagonistic activity of Lactobacilli derived from raw milk

Shwet Nisha*, Kumar Ramashankar & Rashmi Kumari
University Department of Zoology, B.N.M. University, Madhepura, Bihar, India
Received : 15th October, 2021 ; Revised : 16th December, 2021

Abstract– Altogether 15 milk samples were collected from local dairy firm of Madhepura district and Lactobacilli were isolated. Altogether 3 isolates were obtained, LM1, LM2 and LM3 which were identified as Lactobacillus casei, Lactobacillus brevis and Lactobacillus fermentum respectively. The identification were made on the basis of their morphological, biochemical and sugar test as prescribed in Bergey’s manual of systematic Bacteriology (second edition-2001). The antagonistic study of isolated strain was made against four pathogenic bacteria Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Proteus sp.. The inhibition zone against Pseudomonas aeruginosa, Escherichia coli and Proteus sp.was observed maximum by Lactobacillus brevis and against Staphylococcus aureus, the maximum inhibition zone was observed by Lactobacillus fermentum.

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