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Screening of Azotobacter isolate for PGT properties

Navodita Kumaria*, Arun Kumara & Kamlesh Kumarb
aUniversity Department of Zoology, B.N.M. University, Madhepura, Bihar, India
bDepartment of Zoology, B.N.M.V. College, Madhepura, Bihar, India
Received : 07th June, 2021 ; Revised : 09th July, 2021

Abstract– Maize rhizosphere soil was collected from 10 villages of Madhepura district. Bacteria from rhizosphere soil were isolated. Altogether, seven isolates were obtained. All belonging to genus Azotobacter. Isolates were characterized on the basis of their morphological and biochemical characters as described in Bergey’s manual of determinative bacteriology. All isolates were able to solubilize phosphate as tested in PVK and NBRIP medium. The solubilization index ranged from 13.7 to 24.9. The maximum was in MAZ5 and minimum in MAZ6. Siderophore production and HCN production was positive in all isolates. The production of Indole acetic acid varied from 30.55 to 48.62 when 5mM Ltryptophan was supplemented in the medium.

Key words: PVK, NBRIP, Solubilization Index, Siderophore.

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