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Assessment of Nitric oxide scavenging activity and Total antioxidant activity of Chitosan extracted from carapace of freshwater edible crab Sartoriana spinigera

Shiny E.C. Kachhap*
Department of Zoology, Marwari College, Ranchi University, Ranchi, Jharkhand, India
Received : 17th December, 2019 ; Revised : 07th February, 2020

Abstract: Antioxidants are produced by the living body to scavenge the free radicals. But due to increased exposure to undesirable factors such as pollutants, smoking, unbalanced diet, the body needs additional supplementary antioxidants. The study was conducted to observe antioxidative property in one such natural supplement that is chitosan. Chitosan is a cationic polysaccharide produced by N-deacetylation of chitin found in exoskeleton of arthropoda, especially crustacea. In this experiment, Chitosan was extracted from Sartoriana spinigera which is a freshwater edible crab of Jharkhand and its Nitric oxide scavenging activity and total antioxidant activity was studied. Percent of scavenging activity of Chitosan against Nitric oxide anion was found to be 21.95 %, 35.49%, 46.90%, and 66.04% at 0.5 mg/ml, mg/ml, 5 mg/ml and 10 mg/ml concentration respectively. Percent of total antioxidant activity of Chitosan was found to be 29.73 %, 33.78%, 50%, and 62.16 % at 50 µg/ ml, 100 µg/ml, 200 µg/ml and 400 µg/ml concentration respectively.

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