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Effect of fluoride contaminated drinking water on the histology of uterus of mice (Mus musculus)

Arbind Kumara* & Shashibala Kumaria
a*P.G. Department of Zoology, Patna University, Science College Campus, Patna – 800005
Received, 20th December, 2012     ;       Revised: 28th January, 2013

Abstract : Fluoride is an environmental pollutant. Consumption of water containing excess fluoride for a long period of time result in fluorosis. In India, millions of people are affected with fluorosis by drinking naturally fluorinated water up to 38.5 ppm fluoride. Female mice (Mus musculus) weighing 25 to 35 gm were randomly distributed into three group of 10 animals each. Solution of sodium fluoride at the dose 20mg and 40mg/kgbw/day were administered orally to two group of mice for 60 days. The other group served as a control. Normal histology of the control uterus showed basically three layers, outer perimetrium, middle thick myometrium and inner endometrium. The endometrium consisted of uterine glands, blood vessels and stromal cells. The uterine layers were seen disrupted due to fluoride toxicity at the dose of 20mgNaF/kgbw /day for 60 days. In the other group of the mice exposed to 40mg NaF/kgbw/day the uterine layers were observed with loose cellular architecture. In higher magnification blood vessels, uterine glands and stromal cells altered their normal shape and size in endometrium layer. The function of uterus is to develop fetus during pregnancy. The lumen of uterus is the implantation site for blastocyte. Long term exposure of female mice to NaF resulted to the destruction of columnar epithelial cells lining the lumen. These changes may adversely affect the structure of uterus and cause pseudo- pregnancy. These finding may have direct bearing on the females drinking a long term fluoride contaminated water and suffering from fluorosis disease.

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