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Plant regeneration from different explants cultures of Moringa oleifera Lam. (Drumstick)

Neeraja Ojhaa* & K.K.Nagb
*aGossner College, Ranchi University, Ranchi
bDepartment of Botany, Ranchi University, Ranchi.

Received , 15th June, 2012 ;Revised: 10th August, 2012

Abstract : Moringa oleifera Lam. (Drumstick), an important medicinal plant belonging to family Moringaceae is grown for its leaf, flower and fruit which are consumed on a large scare as vegetables. The gum obtained from its bark has curatie potentitality in skin irruptions, earpain and headache, syphilis, diarrhea, epilepsy and hysteria, Oil obtained from seeds is used in acute rheumatism and gout. Keeping in view its various uses, the present investigation aiming at regeneration from different explants viz., young shoot, rachis, leaf, fruit, seeds, epicotyle has been attempted using MS or B5 medium. Effect of only two cytokinins was studied namely KN and BAP for regeneration studies. Root differentiated in the culture of epicotyle and roots. These explants were grown on MS or B5 media supplemented with IBA, NAA, IAA (1-10ppm) cytokinin (BAP) was essential for shoot bud initiation. Shoot buds differentiated within 4-6 weeks.
BAP (2.5 ppm) was most effective in including shoot buds (25% cultures showed shoot buds from callus cultured).
Key words : Regeneration, MS, B5 explants, callus.

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