Rachna Prasada* & U.K. Sinhab
a*Department of Botany, Magadh Mahila College, P.U., Patna, Bihar, India
bDepartment of Botany, Patna University, Patna, Bihar, India.
Received ,6th July, 2012 ;Revised: 29th August, 2012
Abstract : Periwinkle (Catharanthus roseus) has gained unique position in the modern day medicinal arena due to its alkaloids. Important among them are vinblastine and vincristine, the anti-cancer agents. But its low yield per hectare makes it prohibitively expensive. Hence, in-vitro techniques using different types of explants is resorted to. Nodal explants showed 100% callusing after 11 days. The calli were friable, loosely arranged and white with plant growth regulators (PGRS) BAP (0.5 mg/l) and NAA (1.0 mg/l).
Key words : 6-Benzyladenine (BAP). Catharanthus roseus explants. MS medium. Naphthalene acetic acid (NAA).