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Histochemical study of prostate gland of Eutyphoeus waltoni (MICH)

Nirmala Tripathia, Vani Vinayakb & Rittika Pandeyb
aP. G. Department of Zoology, S. K. M. University Dumka, Jharkhand, India
bDepartment of Zoology, Patna University, Patna, Bihar, India
Received 20th June,2011.; Revised 25th August,2011.

Abstract : Earthworm is abundantly found in the soil all over India. Pheretima posthuma and Eutyphoeus waltoni (MICH) are the two different species of commonly called Earthworm, an environment friendly annelida found in North India. The utility of earthworm as environment friendly and natural fertilizer of soil is well accepted by scientists all over the World. Histochemical syudy of Prostate gland of Eutyphoeus waltoni (MICH) commonly found in Patna has been studied to ascertain the intensity of DNA, RNA, Proteins, Peptides, SH and SS bonds. Histochemical investigation for nucleic acid revealed that DNA was relatively feeble than RNA in the internal gland cells and epithelial cells. but DNA was very prominent in the brush border of the cells. Lipid and its derivatives were present in all parts of the section and large lipid droplets were found profusely distributed in the cytoplasm of internal gland cells and epithelial cells. Proteins, peptides, SH and SS bounds were invariably present in all parts of the gland. In the internal gland cells amino acids such as tyrosine, arginine and tryptophane were present, though the latter appeared to be present in traces only. Glycogen and other simple polysaccharides were present in high concentration in addition to mucoid substances. Both acid and alkaline phosphatases gave strong positive reaction.


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