Dharmendra Kumar Sinha•, Nirupam, S. K. Srivastava & A. Nath
Cell Biology and Toxicology Laboratory, Department of Zoology, Patna University, Patna – 800005 Received 11th Jan 2007; Revised 28th Feb. 2007
Abstract : In this investigation two different doses of 3 mg/kg b.w. and 5 mg/kg b.w. endosulfan were administered to Swiss albino mice of three different groups- one control and two experimental• and tissues of liver were isolated from normal and endosulfan exposed mice after 14 and 28 days. After that an antioxidant Emblica 0/firma/us (Amla, 6 mg/kg b. w.) was administered to each endosulfan treated group for 14 and 28 days and then studied under Light Microscope. Endosulfan treated hepatic cells showed abnormalities in the form of vacuolations, disruption of lobular boundary and increase in sinusoidal spaces after 14 days of treatment in both the doses of 3 mg/kg b.w. and 5 b.w. Biliary and hepatocellular proliferation leading to breakage of bile canaliculi, rupture of endothelial wall with severe necrosis in central vein have been observed in the 28 days exposure. Pycnotic and hyperchrornatic nuclei show migration towards ruptured central vein. Hyper autolytic vacuoles were more significant in the 5 mg/kg b.w. endosulfan exposure. Increased hepatic lesions served as bio-indicators of exposure to environmental contaminants. Thus it is quite possible that increased lesions might cause autolysis of hepatic cells ultimately leading to cell death. Amla gave rejuvenating impact on the degenerated hepatic cells due to exposure of endosulfan.