Shiny E.C. Kachhapa* & Nayni Saxenab
aDepartment of Zoology, Marwari College, Ranchi University, Ranchi, Jharkhand, India
bUniversity Department of Zoology, Ranchi University, Ranchi, Jharkhand, India
Received : 19th January, 2024 ; Revised : 24th February, 2024
DOI:-https://doi.org/10.5281/zenodo.14257369
Abstract– Chitosan is deacetylated form of chitin extracted from exoskeleton of crustaceans such as crabs, prawn, and even cell wall of fungi. Chitosan being a hetero-polysaccharide and has proved to be biocompatible and also possesses hypocholesterolemic, anti-oxidative, drug delivery efficiencies. In the present study chitosan -in crude and nanoparticle form- has been extracted from carapace of freshwater crab Sartoriana spinigera, commonly found in Jharkhand, followed by their characterization to study their proper efficiency to perform various important functions. Average weight of crude chitosan extracted from carapace powder was 46.34%, and that of chitosan nanoparticle (by sTPP method) was 44.33%. Characterization by FTIR spectrum showed 7 absorption peaks confirming the structure of crude chitosan and chitosan nanoparticle. Degree of deacetylation of crude chitosan was 88.75% and that of chitosan nanoparticle was 92.25%. Average particle size of crude chitosan and nanoparticle was found to be 398±5 nm and 297.5±2.23 nm respectively. XRD of crude chitosan showed a peak at 2θ around 20° typifying its amorphous and pure structure. XRD of chitosan nanoparticle showed a peak at 2θ around 12°, 20°, 28°, 34° typifying its amorphous and complex structure after extraction by sTPP method. SEM results showed smooth, crystalline structure of both crude and chitosan nanoparticle. On the basis of characterization, it can be said that Chitosan in both crude and nanoparticle form extracted from Sartoriana spinigera can now be used as molecules to study their further efficiencies.
Key words: Chitosan, Nanoparticle, Sartoriana spinigera, Characterization
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