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Isolation of amylase producing bacteria from the gut of Labeo rohita

Madhuri Kumari Das* & Nayni Saxena
University Department of Zoology, Ranchi University, Ranchi, Jharkhand, India
Received : 25th May, 2021 ; Revised : 26th June, 2021

Abstract– In the past few years, advancement has clearly been observed in the field of medicine and life sciences in which microbiology has played a crucial role in the elevation of therapeutic industries, food industry and many more. In the present investigation, bacteria was isolated from the gut of Labeo rohita by spread plate technique using NA media and characterised as Bacillus sp. The amylase producing property of bacteria in vitro was confirmed by using starch agar media. Amylase is a proteinaceous enzyme that helps in the breakdown of starch, amylose and amylopectin that are nothing but the naturally occurring polymers of alpha-glucose. Isolated bacteria were rod shaped and Gram positive having a considerable starch hydrolysing capacity. The isolated bacteria can be used to produce amylase enzyme which can be utilized in digestive supplements, food, textile, paper and detergent industries.

Key words: Amylase enzyme, Gut bacteria, Labeo rohita, Alpha-glucose

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