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Study of purification of hemoglobin in Fasciola gigantica (Trematoda:Fasciolida)

Chandra Mohan*
Department of Zoology, Patna University, Patna, Bihar, India
Received : 22nd April, 2020 ; Revised : 19th May, 2020

Abstract The hemoglobin of Fasciola gigantica a trematode parasite in the bile ductules of the water buffalo Bubalus bubalis was isolated and purified by ammonium sulfate fractionation and gel filtration on Sephadex G-75, and 5.7 fold purification was obtained. Gel filtration revealed two fractions designated as FI and FII. Low A412/A280 ratio for the fraction I, suggest it to be a protein impurity while the fraction II represents the true hemoglobin of the trematode. The trematode hemoglobin shows the characteristic absorption maxima at 410, 540 and 576nm. The molecular masses of Fasciola gigantica hemoglobins are estimated to be ca 17kDa by both gel filtration and SDS- PAGE, suggesting the monomeric nature of this protein. Fasciola gigantica has an n-terminal sequence of the stained, excised and obtained by electroblotting of the SDS-PAGE of the purified hemoglobin in tris-tricine, buffer, pH 8.2. The properties of Fasciola gigantica hemoglobin are in conformity with findings on other trematodes.

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