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Spectrophotometric estimation of total protein of larval gut content of Callosobruchus chinensis Linn. infesting gram and moong

Pushpalata Hansdak* & M. Prasad
Department of Zoology, Ranchi University, Ranchi. Jharkhand, India
Received : 20th May, 2015; Revised : 01st August, 2015

Abstract : The estimation of total protein of the host and pest larval gut content of Callosobruchus chinensis Linn. infesting on legumes like stored grain Gram and Moong .These legumes contains rich protein in themselves which is very important component of the human dietary for their proper build up. The estimation of total protein is done by spectrophotometric (By Lowry’s method) process. The pattern of the different larval instars (I-IV) stages growing on these two host provides the total protein content of the larval instars wise infected host and including the uninfected state of the host.

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