Nayeema Khatoona* & A.K.Choudharya
a*Tissue culture laboratory, University Department of Botany, Ranchi University, Ranchi.
Received , 22nd June, 2014; Revised: 30th July, 2014
Abstract: To promote in vitro callus induction, from leaf explants of Solanum torvum Sw. were inoculated on MS Medium with different concentration of 2,4-D alone and different combination with NAA, BAP, IAA, IBA. The explants were maintained at (25° C ± 1° C) and 16h light cycle. The best calluses develop with IBA (1.0 ppm) in combination with BAP (1.0 ppm). On this combination of hormones light green and soft compact callus was observed. The responses of callus induction with other hormonal combination were negative. The present paper depicts the callus induction in Solanum torvum Sw. in in vitro conditions that provide a novel broad spectrum among researchers as a protocol for further research and investigation in tissue culture of medicinal plant Solanum torvum Sw.