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In-vitro clonal propagation of Lawsonia inermis L. – a medicinal plant

Nazra Paikera* & Kunul Kandira
aUniversity Department of Botany, Ranchi University, Ranchi-834001, Jharkhand, India.

Received 15th December, 2011; Revised 21st February, 2012

Abstract : Lawsonia inermis L. belonging to family Lytharaceae is commonly known as henna, which is recognized in traditional system of medicine.
Standardization of protocol was undertaken for achieving callus mass from different parts of Lowsonia inermis L. Different explants responded differently to chemicals and media they had subjected MS medium supplemented with low concentration of IAA had a tendency to regenerate into shoot and complete planlet but high concentration induced callus formation as well as proliferation. Brown embryogenic callus from leaf explants was observed in media containing IAA. Callus tissue is good source of genetic and karyotypic variability so variants can be regenerated from these genetically variable cells.

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