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Studies on in-vitro multiplication ication in pointed gourd (Trichosanthes dioeca Roxb.) through single-node-cuttings

Vishwa Raj Lala*, A.K. Singhb & Bikash Dasc
aDepartment of Biotechnology, Jamshedpur Womens’ College, Jamshedpur bHorticulture and Agro-Forestry Research Programme, ICAR, Eastern Resion Research Centre, Palandu, Ranchi – 834010

Received 17th November, 2008 ; Revised 27th August, 2009

Abstract : In vitro propagation of Trichosanthes dioeca Roxb.locally known as patal. Being very rich in protein and vitamin A, it has certain medicinal properties and many reports are available regarding its role in circulatory system especially in lowering blood sugar and serum triglycerides (3). The fruits are easily digestible and diuretic in nature. It is also known to have antiulcerous effects (5). Being a dioecious crop, the female plants are of economic importance. Propagation of pointed gourd by seeds not only leads to heterozygosity, but also leads to production of maximum proportion of male plants, which are unproductive. For rapid multiplication of pointed gourd plants, micro propagation holds promise. Standardization of media was done by using different combinations of auxins like IAA (1.0 μM) and IBA (0.2 μM) and Benzyl Adenine (0.5 ppm, 1.0 ppm and 1.5 ppm), alone or in combinations in basal media (Moorashige and Skoog media). In both the genotypes of parwal, rooting and shooting initiated, four days after inoculation. The intensity of root and shoot initiation varied according to cultivar in different media. Between the two female cultivars (Swana Alaukik and Swarna Rekha), Swarna Alaukik was more prolific with regard to root and shoot initiation than Swarna Rekha. However, during later stages, the rate of rooting and shooting was at par. In cv. Swarna Alaukik, the per cent of explant having both root and shoot in case of media containing  IAA+1BA was at par with that containing only BA (1.0 ppm).  However, media containing a combination of IAA 0 μ mol), IBA (0.2 μ mol) and BA (1.0ppm) resulted in the maximum per (82.5%) of plants having roots and shoots.

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