Study on cyto- genotoxic potency of Arsenic trioxide on metaphase chromosome in Bone marrow cells of Mus musculus.

Shalini Bharti^a, Arvind Kumar^b & Arun Kumar^a*
^aUniversity Department of Zoology, B. N. Mandal University Madhepura, Bihar, India.
^bDepartment of Zoology, B.N.M.V. College, B. N. Mandal. University, Madhepura, Bihar, India.

Received : 12^th December, 2023 ; Revised : 16^th January, 2024
DOI:-https://doi.org/10.5281/zenodo.14166633

Abstract– The aim of the present study was to assess the cytotoxic and genotoxic potency of Arsenic on bone marrow cells of Mus musculus. For the research experiment, Adult Mus musculus of the same age group were selected for the research experiment and divided into three groups. After the completion of treatment, slides were prepared by the method of colchicine – hypotonic – aceto- alcohol – flame drying- Giemsa staining technique. For the assessment of mitotic chromosomal aberration 500 well- spread metaphase plates were screened randomly under the compound microscope at 100X (Oil immersion). The data obtained from the control and treated group are expressed as mean % ± SE and ‘t’ test was used to determine by Excel Software and the level of significance is p < 0.05. The total chromosomal abnormality was 2.4 ± 0.68 (Control), 34.2 ± 2.12* (AA-1), and 58.8 ± 2.20** (AA-2) and it is a significant difference to control and treated groups at p< 0.05.

Key words: Arsenic, Bone marrow, Metaphase, Mus musculus, Chromosomal aberrations.

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