Khushboo Tiggaa*, Deepshikha Samdershib & Suhasini Besrab
aDepartment of Zoology, S.P. Mahila College, Dumka, S.K.M.University, Dumka, Jharkhand, India.
bUniversity Department of Zoology, Ranchi University, Ranchi, Jharkhand, India.
Received : 12th December, 2022 ; Revised : 13th January, 2023
Abstract– Alpha (α) amylase is the key hydrolysing enzyme involved in the digestion of carbohydrates. Retardation of starch digestion by inhibition of enzymes such as α-amylase plays a key role in the control of diabetes. Purpose of the study was to investigate in vitro antidiabetic property of chitosan extracted from carapace and chelate legs of freshwater edible crab Sartoriana spinigera using Amylase inhibition assay. The amylase inhibition assay was performed using the chromogenic DNSA method where chitosan was screened for its amylase inhibitory activity in a range of 10μg/mL-100μg/mL. IC50 value was calculated using Liner Regression method. The carapace chitosan and chelate legs chitosan was found to exhibit an inhibitory action on amylase enzyme up to 18.87% and 82.00% respectively at maximum concentration (100μg/ml). Acarbose was used as reference drug. Thus, it can be concluded that the chitosan extracted from carapace and chelate legs of Sartoriana spinigera has in vitro antidiabetic potential. Further studies can be carried out on molecular mechanism of amylase inhibition of chitosan extracted from Sartoriana spinigera, to yield an ecofriendly, cost-effective and easily accessible remedy for diabetes.
Key words: Amylase, IC50, Sartoriana spinigera, Diabetes