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Green fabrication and characterization of silver nanoparticles from leaf and bark extracts of Alstonia sp.

Jyoti Kumari & Tanuja*
Department of Botany, Patliputra University, Patna, Bihar, India
Received : 30th December, 2022 ; Revised :30th January, 2023

Abstract– In this study, leaf extracts of Alstonia scholaris (L.) R.Br (E3)., Alstonia macrophylla Wall (E1), and Alstonia venenata R.Br.(E2) were used to compare by synthesis and characterization of silver nanoparticles from them. Characterization of synthesized nanoparticles were primarily done by significant colour changes and silver nanoparticle characteristic peak of 450 nm in UV-Visible Spectroscopy. Further confirmation were done using FTIR, particles size analysis and zeta potential. Morphology of the synthesized nanoparticles were imaged using Field emission scanning electron microscopy (FE-SEM). Active phytochemical constituents of the leaf extracts of Alstonia scholaris and Alstonia macrophylla were characterized using Gas Chromatography-Mass Spectrometry (GC-MS) method. The silver nanoparticles synthesized from three species of Alstonia showed similar pattern in FTIR spectroscopy with prominent peaks at 3291.33 cm-1 (E1), 3304.77 cm-1 (E3), and 3303.89 cm-1 (E2), 1637.26 cm-1 (E1), 1637.15 cm-1(E3), 1637.17 cm-1 (E2) 1065.84 cm-1 (E1), 1065.86 (E3), 1065.95 (E2) and 591.32 cm-1 (E1) 598.44 cm-1, 585.14 cm-1. Zeta potential and particle size analyzer showed Particle size range of 150- 201nm and their zeta potential ranged from -19 to -22 mV. The GC-MS profiles of Alstonia scholaris and Alstonia macrophylla revealed several common phytoconstituents, proving that identical capping and reducing agents resulted in the emergence of similar peaks among the nanoparticles produced from their plant extract. Some phytoconstituents that were observed in both the plant extracts were lupeol, stigmasterol, campesterol, 2(4H)-Benzofuranone 5, 6, 7, 7a-tetrahydro-4, 4, pentane,3-methyl, 7a trimethyl, (-)-Loliolide, ethane,1- chloro-1-fluoro Neophytadiene, Hexahydrofarnesyl acetone.

Key words: Green synthesis, silver nanoparticles, SEM, DLS, Fourier-transform infrared spectroscopy

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