Oxidative stress and male reproductive dysfunctions with reference to orally administered phthalate compound in Mus musculus

Tanuja^a, Kumari Puja^b, Ravish Kumar^c, J.K Singh^d & Anjali Singh^e*
^aUniversity Department of Botany, Patliputra University, Patna, Bihar, India
^bDepartment of Biochemistry, Magadh University, Bodh-Gaya, Bihar, India
^cInstitute for Innovative Research, Patna, Bihar, India
^dS. S. Hospital and Research Institute, Patna, Bihar, India
^eDepartment of Biotechnology, T.P.S College, Patliputra University, Patna, Bihar, India
Received : 20th May, 2021 ; Revised : 19th June, 2021

Abstract– In order to assess the impact of phthalate diester exposure on oxidative stress and reproductive health of male swiss albino mice Mus musculus, it was orally administered for four, six and eight weeks at a dose of 15 mg/kg/b.wt in the experimental groups of mice. Decrease in the testes weight in phthalate treated group III mice in comparison to normal group I and control group II were observed and the values were statistically very significant (P<0.001) after four, six and eight weeks of treatment. The sperm count in treated mice of group III decreased after four and six weeks of exposure and statistically significant decrease was observed after eight weeks of treatment. The decreased testosterone level was not significant after four weeks of treatment but showed statistically significant decrease after six and eight weeks of treatment as compared to mice of normal groups I & control group II. Statistically significant increase in serum level of MDA was recorded in phthalate treated group III mice after four and eight eighth weeks of the experimental period. In the present study, phthalate induced reproductive toxicity in male mice have been shown to be associated with decreased sperm count, decreased testicular weight and decreased testosterone level.

Key words: phthalate, swiss albino mice, testosterone, sperm, oxidative stress

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