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Determination of chlorpyrifos residue in water and liver and muscle of Clarias batrachus by HPLC with UV detection

Priyanka Kujur*, Deepshikha Samdershi, Haque Sana & Suhasini Besra
University Department of Zoology, Ranchi University, Ranchi, Jharkhand, India
Received : 30th December, 2020 ; Revised : 30thJanuary, 2021

Abstract : The present study was conducted for the determination of chlorpyrifos residues in water and tissue (liver and muscle) of the fish Clarias batrachus by HPLC with UV detection. The fish were exposed to 1/5th sublethal concentration of chlorpyrifos i.e., 0.258 mg/L for 96 hr. Water was collected at 0, 24, 48, 72 and 96 hr after the addition of toxicant to correlate the relationship between the concentration added and the concentration left in water. The liver and muscle tissues were collected after 24, 48, 72 and 96 hr for determination of chlorpyrifos residue accumulated in the tissue. Samples were extracted, cleaned up and purified. There was a decrease of chlorpyrifos residue in water and increase in concentration of chlorpyrifos in liver and muscle with increase in time of exposure, which is a sign of bioaccumulation of the toxicant in the fish through uptake. Also, the accumulation was more in the liver than muscle of the fish.

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