Dinesh Krishna* & Arun Kumar
University Department of Zoology, B.N.M.University, Madhepura, Bihar, India
Received :01st June, 2019 ; Revised : 20th August, 2019
Abstract– In bioethanol production saccharification of biomass to fermentable sugar is an important constraint because the enzyme production costs more and the complications associated with the removal of hearse acid, alkali and salts formed after neutralization. This led to the search for low cost enzyme and its combination with dilute acid to enhance biomass hydrolysis. In this study, the biomass of microalgae was hydrolysed using amylase and cellulase enzymes produced by solid state and submerged fermentation processes. Dilute tetraoxosulphate (VI) acid, crude enzyme complex and a combination of both were used to study saccharification of algal biomass. The highest yield of reducing sugar of 0.63 mg/ml was obtained with the co-combination hydrolysis of acid and enzyme, followed by acid hydrolysis (0.41 mg/ ml) while the least was found with enzyme hydrolysis (0.36 mg/ml).
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