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Histopathological study on the effect of cyclophosphamide on the spleen of adult albino rat

Deepshikha Samdershi*, Khushboo Tigga, Priyanka Kujur & Suhasini Besra
University Department of Zoology, Ranchi University, Ranchi, Jharkhand, India
Received :14th July, 2019 ; Revised : 29th August, 2019

Abstract– Cyclophosphamide is a widely used chemotherapeutic drug which has a range of adverse effects on normal cells also. The present study was aimed to investigate the effect of cyclophosphamide on the spleen of adult male albino rat. Rats were divided into two groups of 6 individual each. Group I (Vehicle Control) rats were treated with distilled water, while group II rats were administered with a single dose of cyclophosphamide (200 mg/kg b.wt) intraperitoneally. After 6 days, rats were evaluated for spleen weight and histology of spleen. Cyclophosphamide administration significantly reduced the absolute and relative weight of spleen as compared to vehicle control group rats (p<0.05). Histopathological examination of spleen showed that cyclophosphamide induced marked alterations manifested by loss of clear distinction between red and white pulp, highly expanded red pulp, reduction in number and cellularity of white pulp, enlargement of central arteriole and presence of megakaryocytes in red pulp region. These findings suggested that cyclophosphamide administration may have toxic effects on the spleen resulting in immunosuppression.

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