Home Biospectra (September 2015) Animal Science Histochemical localization of lipids in the larval instar fore-gut and hind-gut peritrophic membrane of gram beetle Callosobruchus chinensis Linn.

Histochemical localization of lipids in the larval instar fore-gut and hind-gut peritrophic membrane of gram beetle Callosobruchus chinensis Linn.

by mset-bospectra
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Pushpalata Hansdak*
Department of Zoology, Ranchi University, Ranchi. Jharkhand, India
Received : 15th May, 2015; Revised : 27th July, 2015

Abstract : Very little literature is available on the histochemical detection and localization of lipids in the gut of insects particularly those leading a pest life. Although lipids are biochemically high energy structural molecules which prefer to organized membranes, organelles and other important cellular structures in combination with protein yet the amount of lipid deposition in cells has been found to be very poor. Therefore a concentric blue black layer has been observed by Sudan B staining technique diagnostic of bound lipids in the peritrophic membrane of larval instars particularly in the section of foregut and hindgut. The larvae were cultured on the dry legume seeds of White peas and Rajma and reflected gradiant of variation in the lipid deposition.

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