In -vitro antibacterial effect of medicinal plant (Gymnema sylvestre) extract.

Vishwa Raj Lal^a* & Radha Krishna Jha^a
a*’Biotechnology Laboratory, University Department of Botany, Ranchi University , Ranchi, Jharkhand, India.

Received, 11^th November,2013 ; Revised. 20th January 2014.

Abstract: The antibacterial activities of Gymnema sylvestre¹ were selected . The main aim of the research work is to confirm the ethno medicinal claim that Gymnema Sylvestre has got antibacterial activities which could be a better alternative for antimicrobials, if proved to be effective enough². For this we have extracted Gymnema Sylvestre with Petroleum ether then with Chloroform and lastly with metanol, using Soxhlet Apparatus. Antibacterial activity of different extracts were evaluated by “Paper Disc Diffusion Method”³ against Bacillus subtilis, Staphylococcus aureus and Eischerichia Coli using Gentamicin as a standard and DMSO as Control Sample. Zone of inhibition of Bacillus subtilis and Staphylococcus Aureus were found respectively when compared with the result of the Standard, while Extracts had no activity against E.coli. The described method is a rapid, homogenous , cost effective and easy to perform system for screening of new potential antimicrobial agents in drug discovery. So, in future the leaves of this plant can be used for selective infections and can he further explored for isolation of the active principles responsible for antimicrobial property.