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Callus Induction and phytochemical screening of Tinospora cordifolia (WilId.) Miers ex Hook. F. and Thoms.

Anitaa*, Meena Kumaria & H. P. Sharmaa
a*Laboratory of Plant Physiology and Biotechnology, Univ. Dept. of Botany, ICU Ranchi

Received 24th November, 2013 ;Revised: 15th January , 2014

Abstract : It is one of the most versatile rejuvenating shrub also known as ‘Giloya’ in Indian vernacular having many therapeutic applications. Various parts of the plant are being prescribed in Ayurveda and other systems of medicine as a monoherbal or polyherbal preparation. The pharmaceutical significance of this plant is mainly because of the leaves, barks and roots contain various bioactive compounds such as alkaloids, glycosides, lactones, steroids, polysaccharides and aliphatic compounds having various medicinal importance; a few important applications are viz. immunomodulatory or immunostimulatory, anti-neoplastic, anti-oxidant, gastrointestinal and hepatoprotection, and side effects prevention of the cancer chemotherapy. The present investigations were carried out with a view to standardize a protocol for callus induction and to substantiate medicinal properties through phytochemical screening. The nodal explants showed 100% callusing in MS medium incorporated with NAA (6mg/L). In another combination of BAP (0.5mg/L,) with IBA (0.5mg/L) and 2-4-D (0.5 mg/L) callusing was seen on leaf explant (8%), while on nodal segment 100% callusing was observed. Different parts of the plant specially stem and leaves of T. cordifolia are very rich in different types of alkaloids, such as saponins, tannins, alkaloids, steroids, terpenoids, glycosides, resins, phlobatannins, resins, flavonoids, cardioglycosides and alkaloides.

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