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Comparative characterization of five cellulolytic bacterial strains isolated from soil samples of Patna region in Bihar

Pinky Prasada*, Tanujaa, S.Bedib & Satyendra Kumarc
aDepartment of Botany, B.M.D College, Dayalpur, B.R.A Bihar, University, Bihar, India
bDepartment of Botany, Patna Women’ College, Patna, Patna University, Bihar, India
cDepartment of Zoology, S.N.S College, Hajipur, B.R.A Bihar, University, Bihar, India

Received 20th June, 2013; Revised 26th July, 2013

Abstract : Five bacterial strains labeled as BS1, BS2, BS3, BS4 and BS5 isolated from soil samples collected locally from Patna region were selected for the present study. Exoglucanase and endoglucanase activities of the strains were assayed using dinitrosalicylic acid method. The strain BS5 showed highest exoglucanase activity in terms of liberated reducing sugar (7.4 μg/ml) followed by BS1 and BS3 (both releasing 6.8 μg/ml reducing sugar). BS 4 showed slightly more exoglucanase activity (5.7 μg/ml) than BS2 (5.5 μg/ml). The highest endoglucanase activity was exhibited by the strain BS4 (3.0 μg/ml) and least by BS2 (2.2 μg/ml). BS1, BS3 and BS5 possessed similar endoglucanase activity (all releasing 2.7 μg/ml reducing sugar). Colony characteristics and growth adaptability of the strains were investigated. The strains showed moderate to luxuriant growth on different ISP and non ISP media. Good growth was observed at pH 5-11 with optimum being 7 to 9. Slight to luxuriant growth of the selected strains were observed at the temperatures of 15, 26, 37 and 45°C.

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