Narendra Kumar Pandeya*, H. P. Sharmaa & Binod Singha
a*Laboratory of Plant Physiology & Biotechnology, University Department of Botany,
Ranchi University, Ranchi-834008, Jharkhand, India
Received 20th January, 2013 ; Revised 25th February,2013
Abstract : To promote in vitro callus induction, in leaf segments of Achyranthes aspera L. were inoculated on MS medium with different concentrations of 2,4 –D alone and different combinations with NAA ,BAP ,IAA ,IBA. The explants were maintained at (25°C ± 1°C) and 16 h light cycle. The best calluses develop with IBA (1.0 ppm) in combination with BAP (1.0 ppm). On this combination of hormones light green and soft compact callus was observed.The responses of callus induction with other hormonal combinations were negative. The present paper depicts the callus induction in Achyranthes aspera L. in in vitro conditions that provide a novel broad spectrum among researchers as a protocol for further research and investigation in tissue culture of magic herbal plant Achyranthes aspera L.