Inhibitory effect of leaf extract of Ficus hispida L. (Moraceae) on gram +ve bacterium (Bacillus subtilis) and gram -ve bacterium (Escherichia coli)

Rumi Ranjan^a* & Shailendra Kumar Sinha^b
aCentral Institute of Mining & Fuel Research (Digwadih Campus, Dhanbad).
^bDepartment of Zoology, P.K.Roy Memorial College (V.B.U. ), Dhanbad, Jharkhand, India.

Received 7th Feb.,2012; Revised: 17th June., 2012

Abstract : The present study, was set out to explore the antibacterial effect and minimum inhibitory concentration (MIC) of the methanol – chloroform (1:1) extract of the Ficus hispida on both the gram +ve (B.subtilis) and gram -ve (E. coli) bacteria. The result with crude leaf extracts clearly showed the antibacterial effect on both +ve (B.subtilis) and -ve (E.coli) bacteria.
However, the extract solvent methanol-chloroform did not confer any antibacterial property on the growth of the above mentioned bacteria.
Minimum Inhibitory Concentration (MIC) of crude leaf extract of Ficus hispida was Conducted on both the organism (B.subtilis and E.coli).The results were plotted as bar, line diagram and table with crude leaf extract concentrations (mg /ml) on X-axis and zone of inhibition (in mm) on Y-axis to obtain the antibiotic assay and minimum inhibitory zone  of crude leaf extract. There was very little difference in actual inhibitory zone of the two bacteria.
On the basis of the result, antibiotic assay gives equivalent concentration of crude sample is  .037 mg/ml of ampicillin on E. coli & concentration of crude sample is equivalent to .345 mg/ml of ampicillin on B. subtilis. Minimum inhibitory concentration is equal .105 mg/ml on B. subtilis & .027 mg/ml on E. coli.