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Two dimensional TLC analysis of IIIrd & IVth larval instar’s gut content of gram beetle Callosobruchus chinensis Linn.cultured on common gram

Pushpalata Hansdak* & M. Prasad
Dept. of Zoology, Ranchi University, Ranchi, Jharkhand,India.
Received 11th November, 2010; Revised 7th February, 2011

Abstract : Following the standard technique of two dimensional Thin Layer Chromatography (Bobbit and Mike 1982) for the separation of constituent biomolecules of the gut content of IIIrd & IVth Larval Instar of gram beetle, Callosobruchus chinensis cultured on common black gram (Cicer arietinum) compatible results have been obtained with respect to the host biochemical composition, Seven varieties of free amino acids and four of simple sugars (manosaccharides) have been isolated from the IIIrd Larval Instar gut content while nine amino acids and four sugars were separated from that of IVth instar larva growing on the same host. The increased numbers of amino acids isolated from the IVth larval gut content clubbed with the isolation of four important monosaccharides reflect the advanced degree of metabolic physiology helping the growth and development of the larval instars from IIIrd stage. Butanol, acetic acid and Distilled water (BAW)in the proportion of 6:2:2 was used as the separation solvent while 2% alkaline permagamente and 1% ninhydrin solution were used as the location reagent .The glass plate of 4mm thickness in the size of 15×23 cm coated with calcium sulphate mixed silica gel solution was used as the stationary phase in both the dimentions-horizontal & vertical, in the chromatographic analysis.


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