Investigation of inhibition of protein denaturation assay by chitosan extracted from exoskeleton of Sartoriana spinigera (Wood-Mason, 1871)

Khushboo Tigga*^a, Upasana Marandi^b & Suhasini Besra^a
aUniversity Department of Zoology, Ranchi University, Ranchi, Jharkhand, India
^bXavier Institute of Polytechnic and Technology, Ranchi, Jharkhand, India
Received :23^rd October 2018 ; Revised :21^st January 2019

Abstract : Chitosan extracted from exoskeleton (carapace) of freshwater edible crab Sartoriana spinigera (Wood- Mason, 1871)¹ was assessed for in-vitro Protein (BSA) denaturation inhibition. The % Protein Denaturation Inhibitory activity of chitosan was tested at subsequent concentrations of 50 μg/ml, 100 μg/ml, 150 μg/ml, 200 μg/ml and 250 μg/ ml and the present results exhibited a concentration dependent inhibition of protein (BSA) denaturation by the test extract i.e. 42.35±0.08, 49.41±0.9%, 58.82±0.67%, 69.41±0.60% and 83.53±1.03% respectively. Diclofenac Sodium was used as reference drug. 50 % Inhibitory Concentration (IC₅₀) values on protein (BSA) denaturation of Diclofenac sodium was 53.57(μg/ml) while IC₅₀ value of the carapace chitosan was found to be 97.72 (μg/ml).

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