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Antibacterial activity of chitosan of freshwater crab, Maydelliathelphusa masoniana (Henderson, 1893) against Staphylococcus aureus and Escherichia coli.

Pulin Kerketta*, Kumari Neetu & Suhasini Besra
University Department of Zoology, Ranchi University, Ranchi, Jharkhand, India
Received :3rd November 2018; Revised : 1st February 2019

Abstract : Chitosan is known for its diverse functional properties due to its biological activities and application in the pharmaceutical and food industries. Antibacterial properties of chitosan extracted from shell waste of freshwater crab, Maydelliathelphusa masoniana of Jharkhand were determined against Gram negative bacteria, Escherichia coli (ATCC 25922) and Gram positive bacteria, Staphylococcus aureus (ATCC 25923). The antibacterial activity of chitosan (5mg/ ml) in 0.2% acetic acid, was tested by Well-Diffusion method. Zone of inhibition measured for E.coli was 19.33±0.57 mm and 18.66±0.77 mm for S.aureus. Oflaxacin (5mcg) drug showed 31.66±0.57 mm for S.aureus and 29.66±0.57 mm for E. coli. Comparative statistical analysis by Student’s t-test showed that the antibacterial efficacy of chitosan solution was similar both for E.coli and S.aureus. Negative control (0.2% acetic acid) does not show any zone of inhibition. These results indicate that chitosan from shell waste of freshwater crab Maydelliathelphusa masoniana could be used as an effective antibacterial agent against E.coli and S.aureus.

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