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Evaluation of cytotoxic and genotoxic effects of Butyl Hydroxylated Anisole (BHA) on somatic cells of Allium cepa L.

Renu Kathpaliaa*, Largee Biswasb, Kriti Bhardwajc, Kapinderc & Anita Kamra Vermab*
aDepartment of Botany, Kirori Mal College, University of Delhi, Delhi, India
bNanobiotech Lab, Kirori Mal College, University of Delhi, Delhi, India
cDepartment of Zoology, Deen Dyal Upadhyaya Gorakhpur University, Uttar Pradesh, India
Received : 28th December, 2021 ; Revised : 27th January, 2022

Abstract– Food preservatives are intentionally added in small quantities during processing of food to improve the taste quality and shelf-life of the food. Therefore, we are constantly exposed to different food preservatives in daily life that may or may not show adverse effects. Evaluating the cyto-genotoxicity of food preservatives is necessary. Dose dependent and Time dependent effect of Butyl hydroxylated anisole (BHA), commonly used food preservative was done at two concentrations, 0.01mg/L and 0.04mg/L. The mitotic index had been calculated for the control and treated cells. The mitotic indices at 24hrs were 71.67±1.527 for control and 69.33±2.081 and 35.33±1.527, respectively. The mitotic indices at 48hrs were 54.67±2.081 and 27.67±2.081, respectively. The best results were observed in 72 hrs where the mitotic indices were 48.33±2.88 and 18.33±2.88 at 0.4mg/L. Chromosomal abnormalities like multinucleated cells, laggard and anaphase bridges were observed. The effect of preservative BHA on the somatic cells of Allium cepa was cyto-genotoxic.

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