Riti Kumari & B. K. Dayal*
University Department of Botany, B.N. Mandal University, Madhepura, Bihar, India
Received : 2nd June, 2021 ; Revised : 4th July, 2021
Abstract– Procured sample of Adhatoda vasica L. was kept to the laboratory in small pockets of muslin clothes and weighed properly. The samples contained in pockets were stored under 60, 70, 80 and 90%. Relative humidity for different incubation 15, 30, 45 and 60 days. The control was also stored for 60 days. On interval of every 15 days the sample was washed with distilled water thrice and filtered. The filtrates were agar plated on Czapex Dox agar medium. The frequencies of different fungal species in the form of (%) per cent incidence was calculated. The quality of the drug sample was determined in terms of quantity deterioration of different chemical constituents which was estimated by Spectrophotometry method using calibration curve. All together 13 species of fungi were observed. Lowest frequency (% incidence) was formed after 15 days at 70% Relative humidity for Aspergillus ustus as 1.2 while at 90% the percent incidence found was 8.1 for this species. The maximum percent incidence for Aspergillus elagans was observed at 90% Relative humidity after 60 days of incubation as 9.8. Quantitative estimation of sugar, protein, alkaloid, saponin were carried out. The result obtained showed a sharp reduction in the Total sugar, Reducing sugar, Protein and Alkaloid etc. Maximum deterioration was found at 90% R.H. after 60 days of incubation which corresponded to 48% for sugar deterioration. For protein maximum deterioration was found at 90% R.H. after 60 days over control which corresponded to 29%. In the same way maximum deterioration for total alkaloid content showed 34%, while it was 31% for saponin content.
Key words: Relative humidity, Incubation, Fungal incidence, Quality deterioration
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