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Antibacterial activity of chitosan of freshwater crab, Sartoriana spinigera (Wood Mason,1871) against human pathogens

Pulin Kerketta* & Suhasini Besra
University Departement of Zoology, Ranchi University, Ranchi, Jharkhand, India
Received : 22nd November, 2017 ; Revised :26th December, 2017

Abstract :Antibacterial properties of chitosan extracted from shell waste of freshwater crab, Sartoriana spinigera of Jharkhand were determined against Gram-negative bacteria, Pseudomonas aeruginosa (ATCC 27853) and Gram-positive bacteria, Staphylococcus aureus (ATCC 25923). The antibacterial activity of chitosan (5mg/ml) in 0.2% acetic acid , was tested by Well –Diffusion method. Zone of inhibition measured for P. aeruginosa was 16.66±0.57 mm and 18.66± 0.70 mm for S. aureus. Ofloxacin (5mcg) drug showed 31.66±0.57 mm for S. aureus and 24.66±0.57 mm for P. aeruginosa. Statistical analysis by Student’s t-test showed that the antibacterial efficacy of chitosan in S. aureus was significantly more than in P. aeruginosa at 5% level. Negative control (0.2% acetic acid) does not show any zone of inhibition. These results indicate that chitosan from shell waste of freshwater crab Sartoriana spinigera could be used as an effective antibacterial agent against P. aeruginosa and S. aureus.

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