Nuzhat Afroz & Shalini Lal*
Department of Botany, Ranchi College, Ranchi University, Ranchi, Jharkhand, India
Received : 20th June, 2016; Revised: 26th August, 2016
Abstract : An efficient protocol was devised for rapid callus induction and plantlet regeneration from young leaves of Solanum nigrum. For in vitro callus induction auxins such as 2, 4-D, in combination with cytokinin BAP were used. High frequency of green compact callus was obtained in leaf explants cultured on MS medium supplemented with 0.25 mg/l 2, 4-D +2.0 mg/l BAP. The present study also describes successful plant regeneration from in vitro derived callus of young leaves. BAP alone or in combination with 2,4-D was used for regeneration of plantlets from callus culture. High frequency and maximum number of multiple shoots were induced on MS medium supplemented with 2.0 mg/l BAP + 0.25 mg/l 2,4-D.