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Light and Transmission Electron Microscopic studies on the spleen of rat (Rattus norvegicus)exposed to sodium fluoride

Arbind Kumar* & Sapna Kumari
Cell Biology & Toxicology Laboratory, P.G. Department of Zoology, Patna University, Patna
Received 15th April, 2010; Revised 9th August, 2010

Abstract : This study used light and transmission electron microscope to observe the effect of fluoride toxicity on the nodular follicles of white pulp of spleen of albino rat (Rattus norvegicus). The rats were treated with 25mg & 50mg Sodium Fluoride (NaF)/kg/bodyweight/day for 30 days. Light microscopic observation of the NaF treated animals showed the disorganization in the shape of nodular follicles, prominent cracks in the germinal center, eccentrically placed and narrowed central artery and infiltered lymphocytes in the marginal zone area. Transmission electron microscopic observation revealed the damaged endoplasmic reticulum (ER) in the plasma cell of the spleen of treated rats. It was found that the concentric arrangement of ER was disorganized and the number of ER was decreased. The shape of the nucleus of the lymphocyte was mostly elongated and the chromatin profile of the nucleus of the plasma cell was abnormal and damaged. The changes were more pronounced in the animals treated with the higher doses of NaF. These changes observed in lymphocytes and plasma cells might result in abnormal or reduced production of antibodies which in turn may adversely affect the primary immune response against bacterial or pathogenic infections.


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