Bhola Paswan*, Shamshun Nehar, Bushra Raza & Dipali Raha
Department of Zoology, Ranchi University, Ranchi, India
Received 11th Jan. 2007; Revised 25th Feb. 2007
Abstract: Although majority of ovarian follicle undergo atresia a process that exhibit both morphological and biochemical features of programmed cell death or apoptosis. In vivo study concerning the hormonal regulation of atresia have been difficult due to heterogeneous population of follicles in the ovary. In the present study the regulation of apoptosis was examined by IGF-I using a well established method of 24 h culture of ovarian follicle from different reproductive phases in the presence of increasing concentration of the IGF-1(0.01mg/m1, 0.1 mg/mI and 1mg/m1) and Mono as well as oligo-nucleosomal detection was carried out as a quantitative measure of apoptosis. IGF-I was able to suppress onset of DNA fragmentation in both previtellogenic and vitellogenic phase in a dose dependent manner. IGF-I at a dose 0.01mg/m1 supressed DNA fragmentation by 9.5% at 0.1mg/m1 by 18.18% and at 1mg/m1 suppressed DNA fragmentaion by 24.09%. In vitellogenic phase 0.01 mg/ml IGF-I suppressed apoptosis by 20%, 0.1mg/m1 IGF-I suppressed by 32% and 1 mg/ml IGF-I suppressed apoptosis by 52%. This underlines IGF-I acts like a survival factor in different reproductive phase.