Studies on callus induction of Murraya koenigii Spreng in auxin and cytokinin

Nazra Paiker & Kunul Kandir
University Department of Botany, Ranchi University, Ranchi, Jharkhand
Received :8^th November 2018 ; Revised : 18^th February 2019

Abstract : Different concentration of phyto-hormones affected callus formation of Murraya koenigii Spreng was cultured in MS Medium supplemented with different concentration of 2, 4-D, IAA, IBA, NAA, KN and coconut water. Cultures were kept on 25 ± 2⁰C temperature and 16 hour photoperiod while callus was observed on different counteraction of auxin or cytokinin alone or in combination. Most suitable medium for callus formation from shoot tip, leaf internode was that 2,4-D (2.0mg/l), IAA (2.0mg/l), IBA (2.0mg/l), NAA (2.5 mg/l), 2,4-D (2.5mg/l) + IBA (1.5mg/l) + 20% CW, 2, 4-D (1.5mg/l) + KN (0.5mg/l).

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