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Azadirachta indica leaf and bark methanolic and aqueous extracts: phytochemical analysis and antibacterial activity against Escherichia coli

Rachna Bharti & Rimjhim Sheel*
Department of Botany, Ganga Devi Mahila College, Patliputra University, Patna, Bihar, India

Received : 04th December, 2024 ; Revised : 06th January, 2025
DOI:-https://doi.org/10.5281/zenodo.16613052

Abstract– Neem, or Azadirachta indica, is a tropical evergreen tree that grows quickly and is mostly found in America, Africa, and India. It is known in Sanskrit as ‘arishtha,’ meaning ‘perfect, complete, imperishable, and healer of diseases.’ India has supported neem tree scientific study in an attempt to boost business interests and revive traditional customs. Among the phytochemicals identified in the A. indica leaf and bark extracts employed in this study were flavonoids, phenolics, tannins, saponins, alkaloids, and steroids. Phenolics and tannins were present in differing amounts in the methanolic and aqueous extracts. The bark and leaves of A. indica exhibited antibacterial activity against E. coli in both methanolic and aqueous preparations. The aqueous extract had better antibacterial activity than the methanolic extract. The antibacterial activity of A. indica extracts was concentration-dependent, as evidenced by the various zones of inhibition with concentration variations.

Keywords: Azadirachta indica, Escherichia coli, antibacterial, phytochemical

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