Panduranga Murthy G.a, Leelaja B. C.b, Ravishankar H. G.a,b & Prasanjit Mukherjeec
aDepartment of Biotechnology Engineering, PES University, RR Campus, Bengaluru, Karnataka, India.
bDepartment of Studies in Molecular Biology, University of Mysore, Karnataka, India.
cDepartment of Botany, K.O. College, Gumla, Ranchi University, Jharkhand, India
Received : 28th June, 2024 ; Revised : 23rd July, 2024
DOI:-https://doi.org/10.5281/zenodo.15118375
Abstract– Aristolochia indica, L. an Indian Birthwort is a twisting perennial plant with an ancient history of ethnomedicinal use. The focal objective of the study intended to make accessible the contemporary information on the traditional uses of root part (core focus), its chemical constituents, pharmacological importance, antimicrobial efficacy and DPPH radical scavenging activity in the root extract of A. indica. In addition, the rationale was to accentuate the prospective uses of this plant root as a further scope of research in terms of newer phyto-medicine or as a lead factor with a configure of functional drug. Phyto-chemical analyses were done in the root extract of A. indica, then, the buffer extraction from roots of Aristolochia indica, L. was obtained and the protein was concentrated by adding known amount of ammonium sulphate to the extract. Then, the extract was subjected for fractionation through sephadex G-75 Column. As per the accomplished research literature, the diverse chemical compounds including aristolochic acids, aristolactam, phenanthrenes, alkaloids, lignans, steroids and terpenes are revealed in the different parts of the plant. In the present study, the generated data on phytochemical constituents were logically analyzed. Subsequently, in the protein extraction processes, three different peaks of protein, P1, P2 & P3 were obtained and the Peak – 3 samples was subjected for refractionation through the same column. As a result, P4, P5 & P6 were obtained and the protein of highest peak (P5) was directed to assess for different bio-molecules like, total protein, poly phenol and total sugar. Further, the antimicrobial activity of P5 sample which was obtained from the refractionation of Aristolochia root extract was tested against Escherichia coli strain using disc diffusion method. It showed significant inhibitory effect as compared to the standard antibiotic Gentamycin. The preliminary data have confirmed that the most significant activity that supports the traditional use of plant-root as a key component in their drug formula of therapeutic system. The inclusive data pertaining to the dose of crude root extracts and its protein component needs to be purified for ascertaining the drug functionality and its mechanism.
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