Manisha Kumari & Arun Kumar*
University Department of Zoology, B.N. Mandal University, Madhepura, Bihar, India
Received : 19th May, 2024 ; Revised : 19th June, 2024
DOI:-https://doi.org/10.5281/zenodo.15080602
Abstract– Azotobacter sps. are able to fix atmospheric nitrogen. They are considered as plant growth promoting rhizobacteria (PGPR). In the present study, seven isolates of Azotobacter were isolated from rhizospheric soil collected from wheat and maize field. N2 fixation capacity of all isolates was tested at different temperature and pH. Out of 7 isolates 4 belongs to A. chroococcum and 3 belongs to A. vinelandii. All isolates were able to grow in nitrogen free medium and also able to fix nitrogen. Nitrogen fixation capacity of all isolates was maximum at a temperature of 34oC and alkaline pH. Isolate ACW3 of A. chroococcum produced 11μg/g soil at 34oC temperature and pH-8 which was maximum among all isolates. At temperature below 34oC or above it decreased nitrogen production. Similarly at low pH, Nitrogen production decreased.
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