Priyanka Kumari* & Manoj Kumar
Department of Botany, College of Commerce, Arts and Science, Patliputra University, Patna, Bihar, India
Received : 20th June, 2024 ; Revised : 20th July, 2024
DOI:-https://doi.org/10.5281/zenodo.15106388
Abstract– In the realm of advanced molecular genetic analysis, techniques such as phylogenetic analysis, marker-assisted selection, purity analysis, gene mapping, and DNA fingerprinting, heterotic analysis play a vital role. However, one of the most critical and cumbersome steps in these processes is the high-throughput DNA isolation process. This step is not only labor-intensive but also time-consuming, often serving as a bottleneck in molecular genetic analysis. This study introduces a simplified, cost-effective modified CTAB-DNA isolation method that can be efficiently executed in a laboratory with basic equipment. Notably, this approach eliminates the need of liquid nitrogen and hazardous chemical like β-mercaptoethanol, thereby enhancing laboratory safety and cost-effectiveness. The amount and quality of isolated Deoxyribonucleic acid (DNA) depends on starch and non-cellulosic occurrence in the leaf wall of Musa paradisiaca (Banana). However, the described method overcomes these challenges, yielding high-quality DNA well-suited for PCR analysis. This modified CTAB-DNA isolation approach offers a rapid, easy, inexpensive, and reliable solution for researchers working on banana, making it an attractive option for molecular genetic studies.
Be First to Comment