Akash Garain* & Rajendra Mistry
University Department of Zoology, V.B. University, Hazaribag, Jharkhand, India
Received : 23rd April, 2023 ; Revised : 24th May, 2023
DOI:-https://doi.org/10.5281/zenodo.10690016
Abstract– The effect of hydrocortisone acetate and L-thyroxine on blood volume and haematocrit values in Mystus bleekeri of almost same age group (23 – 35 grams) was studied at 28°C. The blood volume was determined by dye dilution technique using Evans blue. Different doses (total viable dose 1 and 3 mg/ml/100 g of hydrocortisone acetate and 0.5 and 1 mg/ml/100 g of L-thyroxine) of hormones were injected intraperitoneally in a period of 10 days (daily) in different sets of animals. Saline injected control animals had a total blood volume of 0.378 ± 0.051 ml, relative (per 100 g body weight) blood volume of 1.643 ± 0.221 ml and haematocrit value of 45.0 ± 1.22%. Hydrocortisone dose of 1 mg/100 had TBV of 0.278 ± 0.028 ml. RBV of 1 108 ± 0.113 ml and Ht value of 44.0 ± 0.96% Hydrocortisone dose of 3.0 mg/ml/100g had TBV of 0.222 ± 0.021 ml, RBV of 0.965 ± 0.090 ml and Ht value of 33.0 + 0.84″. L-thyroxine treated animals (0.5 mg/ 100 g) had TBV of 0.417 ± 0.02 ml, RBV of 1.737 ± 0.125 ml and Ht value of 25.0 ± 0.75% L-thyroxine dose of 10W/100 had TBV or 0.253 0.026 ml, RBV of 1.100 = 0.117 ml and Tilt value of 28.0 ± 0.71%. From t-test of the data the value of “P” shows that in case of hydrocortisone injected animals the total and relative blood volume as well as haematocrit values become less in both the doses but it is more significant (P<0.001, 0.05 and 0.001) in higher dose. In case of L-thyroxin injected animals the results were somewhat different in different doses. In smaller dose total and relative blood volumes were increased (N S.) but the haematocrit value was decreased, whereas in higher dose blood volumes and the value of haematocrit were significantly decreased (P<0.005, 0.005 and 0.001). From the experiment it is inferred that both the hormones reduce blood volume and the haematocrit values in this air breathing fish (Mystus bleekeri).
Key words: Water quality index, river systems, Jharkhand state
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